Host: Rabbit
Target Protein: Peptidylprolyl Isomerase A
Specificity: Knockdown Validated
Immunogen Range: 180-224/224
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 5478
Swiss Prot: P62937
Source: A synthesized peptide derived from human Cyclophilin A
Purification: Affinity Purified
Storage Buffer: Supplied in PBS (pH 7.4) containing 50% glycerol and 0.02% sodium azide.
Storage: -20 °C
Background:
Size: 50 ul Ab + 25ug cell Lysate + 25ug WT cell,50
Concentration: Lot dependent
Predicted Molecular Weight: 18 kDa
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
Flow cytometric analysis of Peptidylprolyl isomerase A expression in HepG2 cells using anti-Peptidylprolyl isomerase A antibody (1:2,000). Green, isotype control; red, Peptidylprolyl isomerase A.
Immunocytochemical staining of HepG2 cells with anti-Peptidylprolyl isomerase A antibody (1:1,000). Nuclei were stained blue with DAPI; Peptidylprolyl isomerase A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-Peptidylprolyl isomerase A antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Peptidylprolyl isomerase A antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit.