Host: Rabbit
Target Protein: Paxillin
Specificity: Knockdown Validated
Immunogen Range: 100-165/165
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 5829
Swiss Prot: P49023
Source: A synthesized peptide derived from human Paxillin
Purification: Affinity Purified
Storage Buffer: Supplied in PBS (pH 7.4) containing 50% glycerol and 0.02% sodium azide.
Storage: -20 °C
Background:
Size: 50 ul Ab + 25ug cell Lysate + 25ug WT cell,50
Concentration: Lot dependent
Predicted Molecular Weight: 65 kDa
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Immunocytochemical staining of Hela cells with anti-Phospho-Paxillin (Y118) antibody (1:1,000). Nuclei were stained blue with DAPI; Phospho-Paxillin (Y118) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-Phospho-Paxillin (Y118) antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Paxillin (Y118) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit.
Western blotting analysis using anti-Phospho-Paxillin (Y118) antibody. Phospho-Paxillin (Y118) expression in wild type (WT) and Phospho-Paxillin (Y118) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulinserves as a loading control. The blot was incubated with anti-Phospho-Paxillin (Y118) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit.