Host: Rabbit
Target Protein: PC4 And SRSF1 Interacting Protein 1
Specificity: Knockdown Validated
Immunogen Range: 40-92/637
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 11168
Swiss Prot: O75475
Source: A synthesized peptide derived from human PSIP1 / LEDGF
Purification: Affinity Purified
Storage Buffer: Supplied in PBS (pH 7.4) containing 50% glycerol and 0.02% sodium azide.
Storage: -20 °C
Background:
Size: 50 ul Ab + 25ug cell Lysate + 25ug WT cell,50
Concentration: Lot dependent
Predicted Molecular Weight: 60 kDa
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Flow cytometric analysis of PSIP1 expression in HeLa cells using PSIP1 antibody (1:2,000). Green, isotype control; red, PSIP1.
Immunocytochemical staining of Hela cells with anti-PSIP1 antibody (1:1,000). Nuclei were stained blue with DAPI; PSIP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-PSIP1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSIP1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit.
Western blotting analysis using anti-PSIP1 antibody. PSIP1 expression in wild-type (WT) and PSIP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-PSIP1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit.