DATASHEET
Host:
Rabbit
Target Protein:
Ubiquitin Specific Peptidase 7
Specificity:
Knockdown Validated
Immunogen Range:
100-150/1102
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
7874
Swiss Prot:
Q93009
Source:
A synthesized peptide derived from human HAUSP / USP7
Purification:
Affinity Purified
Storage Buffer:
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage:
-20 °C
Background:
Hydrolase that deubiquitinates target proteins such as FOXO4, DEPTOR, KAT5, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, KMT2E/MLL5 and DAXX. Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis. Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Deubiquitinates KMT2E/MLL5 preventing KMT2E/MLL5 proteasomal-mediated degradation. nvolved in cell proliferation during early embryonic development. Involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage: recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, preventing UV-induced degradation of ERCC6.
Cell Line: HeLa
Knockdown technology: shRNA Knockdown