DATASHEET
Host:
Rabbit
Target Protein:
Histone Acetyltransferase 1
Specificity:
Knockdown Validated
Immunogen Range:
200-419/419
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
8520
Swiss Prot:
O14929
Source:
A synthesized peptide derived from human KAT1 / HAT1
Purification:
Affinity Purified
Storage Buffer:
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage:
-20 °C
Background:
Histone acetyltransferase that plays a role in different biological processes including cell cycle progression, glucose metabolism, histone production or DNA damage repair. Coordinates histone production and acetylation via H4 promoter binding. Acetylates histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, histone H2A at 'Lys-5' (H2AK5ac). Drives H4 production by chromatin binding to support chromatin replication and acetylation. Since transcription of H4 genes is tightly coupled to S-phase, plays an important role in S-phase entry and progression. Promotes homologous recombination in DNA repair by facilitating histone turnover and incorporation of acetylated H3.3 at sites of double-strand breaks. In addition, acetylates other substrates such as chromatin-related proteins. Acetylates also RSAD2 which mediates the interaction of ubiquitin ligase UBE4A with RSAD2 leading to RSAD2 ubiquitination and subsequent degradation.
Cell Line: HeLa
Knockdown technology: shRNA Knockdown