DATASHEET
Host:
Rabbit
Target Protein:
DNA-dependent protein kinase catalytic subunit
Specificity:
Knockdown Validated
Immunogen Range:
2049-2063/4128
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
5591
Swiss Prot:
P78527
Source:
A synthesized peptide derived from human Phospho-DNA PKcs (S2056)
Purification:
Affinity Purified
Storage Buffer:
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage:
-20 °C
Background:
Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). Recruited by XRCC5 and XRCC6 to DNA ends and is required to 1 protect and align broken ends of DNA, thereby preventing their degradation, 2 and sequester the DSB for repair by NHEJ. Act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion (By similarity). Also involved in modulation of transcription. As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome. Binding to U3 small nucleolar RNA, recruits PRKDC and XRCC5/Ku86 to the small-subunit processome. Recognizes the substrate consensus sequence [ST]-Q.
Cell Line: HeLa
Knockdown technology: shRNA Knockdown