bsm-90009R

DATASHEET

Host: Rabbit

Target Protein: Minichromosome Maintenance Complex Component 6

Specificity: Knockdown Validated

Immunogen Range: 601-821/821

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 4175

Swiss Prot: Q14566

Source: A synthesized peptide derived from human MCM6

Purification: Affinity Purified

Storage Buffer: Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.

Storage: -20 °C

Background:

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

Cell Line: Hela

Knockdown technology: shRNA Knockdown

Size: 50 ul Ab + 25ug KD cell Lysate + 25ug WT cell

Concentration: Lot dependent

Predicted Molecular Weight: 93 kDa

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.