bsm-70713M

DATASHEET

Host: Mouse

Target Protein: VASP (C-terminal region)

Specificity: This antibody detects 46 and 50 kDa* proteins corresponding to the apparent molecular mass of VASP on SDS-PAGE immunoblots of human A431, HeLa, and HUVEC.

Clonality: Monoclonal

Isotype: IgG1

Swiss Prot: P50552

Source: Clone (M277) was generated from a recombinant protein that includes amino acids from the C-terminal region of human VASP.

Purification: Purified by Protein A.

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Actin filament tethering and bundling are important mechanisms involved in actin superstructure assembly. The ENA/VASP family includes VASP, mena, and Ena-Vasp-like (EVL). These multidomain proteins localize to the leading edge of filopodia where they associate with AFs, interact with profilin, and compete with capping proteins at the barbed end of AFs. Artificial relocalization of VASP from the plasma membrane to mitochondrial membranes inhibits filopodial formation and axon branching, while deletion of all three ENA/VASP proteins produces defects in cortical axon-tract formation. Regulation of VASP protein activity occurs through phosphorylation at Ser-157, Ser-239, and Thr-278. AMPK phosphorylates Thr-278, leading to impaired actin stress fiber assembly and changes in cell morphology.