bsm-70684M

DATASHEET

Host: Mouse

Target Protein: Stat1

Specificity: The antibody detects 84 and 91 kDa* stat1 variants on SDS-PAGE immunoblots of human Jurkat and A431 cells.

Clonality: Monoclonal

Isotype: IgG1

Swiss Prot: P42224

Source: Clone (M249) was generated from a recombinant protein from human Stat1. This sequence has high homology to the conserved region in rat and mouse stat1 a and b.

Purification: Purified by Protein A.

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat1 is expressed as two variants of 84 and 91 kDa. Stat1 proteins contain SH2 and SH3 domains, and are components of the interferon-stimulated gene factor 3 (ISGF3) complex. This complex is the primary transcription activator induced by the binding of interferon to its receptors. In response to activation by various cytokines and growth factors, stat1 subunits become phosphorylated at tyrosine 701. This leads to translocation of stat1 to the nucleus, resulting in formation of an active ISGF3 complex. Active ISGF3 modulates the transcription of the interferon-stimulated genes. Thus, phosphorylation of Tyr-701 is critical for gene expression mediated by various cytokines and growth factors.