bsm-70663M

DATASHEET

Host: Mouse

Target Protein: SHP2 (N-terminal region)

Specificity: The antibody detects a 72 kDa* protein in human A431 and Jurkat cells, and mouse brain. This antibody does not cross-react with SHP1.

Clonality: Monoclonal

Isotype: IgG1

Swiss Prot: Q06124

Source: Clone (M163) was generated from a recombinant protein containing amino acids in the N-terminal region of human SHP2. This sequence is highly conserved in rat and mouse SHP2.

Purification: Purified by Protein A.

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

SHP2 (PTP1D, SH-PTP2, or Syp) is a widely expressed protein-tyrosine phosphatase (PTP) that maintains phosphotyrosine homeostasis during growth factor, cytokine, hormone and antigen receptor signaling. This phosphatase contains two N-terminal SH2 domains and a C-terminal phosphatase domain. SHP2 associates with EGF and PDGF growth factor receptors and is activated after stimulation of these receptors. Activation of SHP-2 and its association with Gab1 is critical for sustained ERK activation downstream of both growth factor and cytokine receptors. In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 p85 binding sites. Thus, SHP2 is critical for maintaining phosphotyrosine homeostasis in many cell signaling pathways