bsm-70513M

DATASHEET

Host: Mouse

Target Protein: Anti-His (C-terminal) Tag Antibody

Specificity: Clone M050 detects recombinant proteins with C-terminal His-tag epitope. The antibody binds 6 and 10 His-tag epitopes on the C-terminal side of a protein, but does not bind N-terminal His-tag epitopes. The antibody works for western blot, immunoprecipitation, immunocytochemistry, flow cytometry, and ELISA capture.

Clonality: Monoclonal

Isotype: IgG1

Swiss Prot: N/A

Source: Clone (M050) was generated from a recombinant protein containing a C-terminal His-tag.

Purification: Purified by Protein G.

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Epitope tagging (e.g. His-tag) is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). A variety of plasmids contain DNA that encodes an amino- or carboxy-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. These plasmids facilitate expression of His-tagged recombinant proteins that can be isolated or purified by immobilized metal affinity chromatography. Anti-His Tag antibodies can be used to detect recombinant proteins with the His-Tag in many different immunoassays, including western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry, flow cytometry, ELISA, and chromatin immunoprecipitation assays.