bsm-70510M

DATASHEET

Host: Mouse

Target Protein: Grb2

Specificity: This antibody detects a 24 kDa* protein corresponding to the molecular mass of Grb2 on SDS-PAGE immunoblots of rat PC12.

Clonality: Monoclonal

Isotype: IgG1

Swiss Prot: P62994

Source: Clone (M331) was generated from a full length recombinant rat Grb2 protein. This sequence has high homology to human and mouse Grb2.

Purification: Purified by Protein A.

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Crk and Grb2 family adaptor proteins are involved in a variety of cell signaling pathways related to human diseases. These adaptors have Src homology 2 (SH2) and Src homology 3 (SH3) domains, which are docking sites for several signaling proteins, including receptors, kinases, and GTPase regulators. In addItion, other protein-protein interactions may also utilize Crk and Grb2 domain interactions to modulate cell signaling pathways. The DOCK family of proteins (DOCK3, DOCK4, and DOCK5) can interact with Crk and may be important Crk effector proteins. The cell cycle regulator p27Kip1 can interact with Grb2, and this implicates Grb2 activity in cell signaling pathways that alter cell cycle progression.