bsm-70409M

DATASHEET

Host: Mouse

Target Protein: CD147/Emmprin/Basigin (Extracellular region)

Specificity: Clone M036 mouse monoclonal antibody detects a 38-58 kDa* protein on SDS-PAGE immunoblots of human BEAS, NCIH28, NCI-H1299, LNCaP, and A431 cells, as well as human brain tissues. The antibody works for western blot, immunoprecipitation, ELISA capture, and immunocytochemistry.

Clonality: Monoclonal

Isotype: IgG2a

Swiss Prot: P35613

Source: Clone (M036) was generated from a proprietary antigen related to the native human CD147 expressed in NCI-H1915 lung cancer cell line.

Purification: Purified by Protein G.

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

D147 (Basigin/EMMPRIN) is a type I integral membrane receptor protein belonging to the immunoglobulin superfamily. CD147 is a glycosylated protein with four known isoforms with isoform 2 being the most abundantly expressed. In many cancers, CD147 is overexpressed and stimulates the secretion of extracellular matrix metalloproteinases by tumor fibroblasts, which can promote tumor progression. CD147 is co-expressed with the amino acid transporter, CD98h, in metabolically active cells such as lymphocytes, macrophages, and tumor cells. CD147 interaction with caveolin and cyclophilins regulates its functions, and this protein may function outside of tumor cells through cell shedding and microvesicle release.