bsm-70405M

DATASHEET

Host: Mouse

Target Protein: CD63 (Extracellular region)

Specificity: Clone M034 mouse monoclonal antibody detects a 30-60 kDa* protein on SDS-PAGE "Native" immunoblots of human A549, MeWo, MDA-MB-231, and MCF7 cells, as well as human breast, lung, and skin tissues. This antibody does not detect denatured CD63. The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry.

Clonality: Monoclonal

Isotype: IgG1

Swiss Prot: P08962

Source: Clone (M034) was generated from a proprietary antigen related to the native human CD63 expressed in MeWo melanoma cell line.

Purification: Purified by Protein G.

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Tetraspanins comprise a large superfamily of cell surface-associated membrane proteins with four transmembrane domains. On cell membranes, tetraspanins form networks of various proteins called tetraspanin-enriched microdomains (TEMs). CD63 was the first characterized tetraspanin and it is found in TEMs, as well as late endosomes and lysosomes. In late endosomes, CD63 is enriched on the intraluminal vesicles, and can be secreted as exosomes through fusion of endosomes with the plasma membrane. The complex localization pattern of CD63 suggests that its intracellular trafficking and distribution must be tightly regulated. CD63 contains N-link glycosylation sites that produce diverse CD63 molecules that range from 30 to 60 kDa depending on cell type. CD63 is an important exosomal marker in cancer cells, and may be involved in cancer progression and metastasis.