bsm-70402M

DATASHEET

Host: Mouse

Target Protein: CD55 (Extracellular region)

Specificity: Clone M033 was purified using Protein G chromatography. The antibody. The antibody also detects a "native" human recombinant CD55 protein that includes the extracellular region. The antibody can be used for native western blot, immunoprecipitation, protein ELISA, and immunocytochemistry, as well for detecting CD55 in live, unfixed cells.

Clonality: Monoclonal

Isotype: IgG1

Swiss Prot: P08174

Source: Clone M033 was generated from a proprietary antigen related to the extracellular region of human CD55 from the MDA-MB-231 breast cancer cell line.

Purification: Purified by Protein G.

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

CD55, also known as Decay-accelerating factor (DAF) is an inhibitor of the complement system, and is broadly expressed in malignant tumours. In cancer, CD55 has been implicated in tumorigenesis, neoangiogenesis, and metastasis. CD55 may decrease complement mediated tumor cell lysis, inhibit tumor apoptosis, and promote invasive cancer cell motility. These roles in cancer may involve binding to the seven-span transmembrane receptor CD97. In neuroblastoma cells, CD55 contributes to growth of colonies and to invasion of cells, but not to stemness. In neuroblastoma cells, CD55 is upregulated in a small population of cells that are HIF-2α positive. This CD55 positive subpopulation is highly invasive and has low adhesion to fibronectin and collagen. In addition, CD55 expression correlates with poor prognosis in neuroblastoma patients.