bsm-70373M

DATASHEET

Host: Mouse

Target Protein: Caspase-3 (N-terminal region)

Specificity: The antibody detects a 32 kDa* protein corresponding to the molecular weight of Caspase-3 on SDS-PAGE immunoblots of human Jurkat and K562 cells.

Clonality: Monoclonal

Isotype: IgG2a

Swiss Prot: P42574

Source: Clone (M377) was generated from a recombinant protein containing amino acid residues in the N-terminal region of human Caspase-3.

Purification: Purified by Protein A.

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The caspases are a group of cysteine enzymes, which cleave proteins in response to intrinsic and extrinsic pathways that cause apoptotic cell death. The caspases can be grouped into two subgroups based on their roles in apoptosis. Initiator caspases (caspases 2, 8, 9, and 10) are activated through the apoptosis-signaling pathways and activate the effector caspases (caspases 3, 6, and 7) which carry out apoptosis. Caspase cascades are initiated through assembly of multiprotein complexes that trigger activation of the initiator caspases, which are then released and are able to activate the downstream effector caspases.