DATASHEET
Host:
Mouse
Target Protein:
Peripherin
Specificity:
Specific for endogenous levels of the ~57 kDa peripherin protein. This antibody performs well on aldehyde fixed tissues.
Clonality:
Monoclonal
Isotype:
IgG1
Entrez Gene:
3414
Swiss Prot:
P21807
Source:
Recombinant rat peripherin expressed in and purified from E. coli.
Purification:
Concentrated tissue culture supernatant
Storage Buffer:
Concentrated tissue culture supernatant + 10 mM NaN3
Storage:
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliq
Background:
Peripherin is a ~57 kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems, which gives the protein its name. Subsequently, peripherin was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which also includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. In contrast to the neurofilaments, peripherin is strongly up-regulated after nerve injury (1). Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig's disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors (2). Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes (3). Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration.