bsm-70168M

DATASHEET

Host: Mouse

Target Protein: Nestin

Specificity: Specific for endogenous levels of the ~220-240 kDa nestin doublet.

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 10763

Swiss Prot: P48681

Source: Recombinant human Nestin expressed in and purifeid from E. coli.

Purification: Purified by Protein G.

Storage Buffer: PBS + 50% glycerol and 5 mm NaN3

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells (Lendahl et al., 1990). Nestin was originally identified as a result of the production of a series of monoclonal antibodies directed against epitopes expressed on formalin fixed embryo day 15 rat spinal cord (Hockfield et al., 1985)). One of these antibodies, called Rat 401, stained fibrous profiles in the developing nervous system, but not in the mature nervous system. By screening bacteriophage expression libraries with Rat 401, Lendahl et al. were able to isolate a cDNA encoding the protein to which Rat 401 antibody bound. The protein proved to be an unusual member of the intermediate filament family, containing an alpha-helical region homologous to that found in keratin and neurofilament subunits.

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 220-240

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.