bsm-70054M

DATASHEET

Host: Mouse

Target Protein: CRISPR Cas9

Specificity: Specific for the Staphylococcus aureus Cas9 protein.

Clonality: Monoclonal

Isotype: IgG1

Source: Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus.

Purification: Purified by Protein G.

Storage Buffer: PBS + 50% glycerol and 5 mm NaN3

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

CRISPR/Cas9 is a genome editing tool that makes possible the permanent alteration of genes by directing specifically designed RNA sequences complexed with the nuclease Cas9 to cleave DNA at any desired location allowing the deletion or addition of genes. Antibodies against Cas9 are very useful in the direct detection of the CRISPR/Cas9 complex. This antibody detects Cas9 from Staphylococcus aureus transfected into cells and shows no reactivity toward the Streptococcus pyogenes Cas9 protein (Ran et al., 2015).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 53

Cross Reactive Species: Others

For research use only. Not intended for diagnostic or therapeutic use.