Host: Rabbit
Target Protein: PAK1/2/3
Modification Site: Ser144/Ser141/Ser154
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 5058
Swiss Prot: Q13153,Q13177,O75914
Source: KLH conjugated synthetic peptide derived from human PAK1 around the phosphorylation site of S144
Purification: Purified by Protein A.
Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: Lot dependent
Predicted Molecular Weight: 58-62
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Monoclonal Antibody, Unconjugated(bsm-62927R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Monoclonal Antibody, Unconjugated(bsm-62927R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Monoclonal Antibody, Unconjugated(bsm-62927R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Monoclonal Antibody, Unconjugated(bsm-62927R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
MCF-7 (H) cells were treated with or without EGF (1µg/ml) for 10 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) monoclonal antibody, unconjugated (bsm-62927R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.