bsm-62900R [Primary Antibody]
Phospho-MEK1 (Thr292) Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MEK1

Modification Site: Thr292

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 5604

Swiss Prot: Q02750

Source: KLH conjugated synthetic peptide derived from human MEK1 around the phosphorylation site of T292

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules.

Size: 100ul

Concentration: Lot dependent

Predicted Molecular Weight: 43


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot analysis of (1) HeLa cell lysate; (2) HeLa cell treated with Nocodazole. Using Phospho-MEK1 (Thr292) (bsm-62900R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Jurkat (H) cells were treated with or without calyculin A (100 nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-MEK1 (Thr292) monoclonal antibody, unconjugated (bsm-62900R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.