bsm-62846R [Primary Antibody]
Integrin alpha V Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Integrin Alpha V

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 3685

Swiss Prot: P06756

Source: KLH conjugated synthetic peptide derived from human Integrin alpha V

Purification: Purified by Protein A.

Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling.

Size: 100ul

Concentration: Lot dependent

Predicted Molecular Weight: 116


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot analysis of (1) A549 cell lysate; (2) Mouse spleen lysate; (3) Rat brain lysate. Using Integrin Alpha V (bsm-62846R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


25 ug total protein per lane of various lysates (see on figure) probed with Integrin Alpha V monoclonal antibody, unconjugated (bsm-62846R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Invasive urothelial carcinoma of the bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin Alpha V Monoclonal Antibody, Unconjugated(bsm-62846R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin Alpha V Monoclonal Antibody, Unconjugated(bsm-62846R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Placenta ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin Alpha V Monoclonal Antibody, Unconjugated(bsm-62846R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.