bsm-62840R [Primary Antibody]
Phospho-STK4/3 (Thr183/Thr180) Recombinant Antibody
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: STK4/3

Modification Site: Thr183/Thr180

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 6789

Swiss Prot: Q13043, Q13188

Source: KLH conjugated synthetic peptide derived from human STK4 around the phosphorylation site of T183

Purification: Purified by Protein A.

Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Size: 100ul

Concentration: Lot dependent

Predicted Molecular Weight: 56


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Ramos (H) cells were treated with or without Staurosporine (1μM) for 1h, Hela (H) cells were treated with or without Calyculin A (50nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-STK4/3 monoclonal antibody, unconjugated (bsm-62840R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.