Host: Rabbit
Target Protein: IRF4
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 3662
Swiss Prot: Q15306
Source: KLH conjugated synthetic peptide derived from human IRF4
Purification: Purified by Protein A.
Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: Lot dependent
Predicted Molecular Weight: 52
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Western blot analysis of Daudi cell lysate. Using IRF4 (bsm-62837R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IRF4 Monoclonal Antibody, Unconjugated(bsm-62837R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IRF4 Monoclonal Antibody, Unconjugated(bsm-62837R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IRF4 Monoclonal Antibody, Unconjugated(bsm-62837R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.