bsm-62534R [Primary Antibody]
HIF-1 Alpha Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HIF-1 Alpha

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 3091

Swiss Prot: Q16665

Source: KLH conjugated synthetic peptide derived from human HIF 1 alpha

Purification: Purified by Protein A.

Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Functions as a master transcriptional regulator of the adaptive response to hypoxia.Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia.

Size: 100ul

Concentration: Lot dependent

Predicted Molecular Weight: 93


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot analysis of HeLa treated with CoCl2 cell lysate. Using HIF-1 Alpha (bsm-62534R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.