bsm-61677R [Primary Antibody]
SHC1 Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SHC1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 6464

Swiss Prot: P29353

Source: KLH conjugated synthetic peptide derived from human SHC1

Purification: Purified by Protein A.

Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Shc1 IS an adaptor protein containing a SH2 domain and a PID domain within a PH domain-like fold. Three isoforms(p66, p52 and p46), produced by alternative initiation, variously regulate growth factor signaling, oncogenesis and apoptosis.

Size: 100ul

Concentration: Lot dependent

Predicted Molecular Weight: 63


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot analysis of MCF7 cell lysate. Using SHC1 (bsm-61677R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


25 ug total protein per lane of various lysates (see on figure) probed with SHC1 monoclonal antibody, unconjugated (bsm-61677R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.