bsm-61648R [Primary Antibody]
Lamin B2 Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Lamin B2

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 84823

Swiss Prot: Q03252

Source: KLH conjugated synthetic peptide derived from human Lamin B2

Purification: Purified by Protein A.

Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

Size: 100ul

Concentration: Lot dependent

Predicted Molecular Weight: 70


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot analysis of (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate. Using Lamin B2 (bsm-61648R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


25 ug total protein per lane of various lysates (see on figure) probed with Lamin B2 monoclonal antibody, unconjugated (bsm-61648R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.