bsm-61622R [Primary Antibody]
Phospho-CRK (Tyr221) Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CRK (Tyr221)

Modification Site: Tyr221

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 1398

Swiss Prot: P46108

Source: KLH conjugated synthetic peptide derived from human CRK around the phosphorylation site of Y221

Purification: Purified by Protein A.

Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.

Size: 100ul

Concentration: Lot dependent

Predicted Molecular Weight: 34


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot analysis of (1) K562 cell lysate treated with AP; (2) K562 cell lysate. Using Phospho-CRK (Tyr221) (bsm-61622R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.