bsm-61331R [Primary Antibody]
CDC25C Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cdc25C

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 995

Swiss Prot: P30307

Source: KLH conjugated synthetic peptide derived from human CDC25C

Purification: Purified by Protein A.

Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle.When phosphorylated, highly effective in activating G2 cells into prophase.Directly dephosphorylates CDK1 and activates its kinase activity.

Size: 100ul

Concentration: Lot dependent

Predicted Molecular Weight: 53


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot analysis of K562 cell lysate. Using Cdc25C (bsm-61331R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


25 ug total protein per lane of various lysates (see on figure) probed with Cdc25C monoclonal antibody, unconjugated (bsm-61331R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.