bsm-61217R [Primary Antibody]
ATG7 Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ATG7

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 10533

Swiss Prot: O95352

Source: KLH conjugated synthetic peptide derived from human ATG7

Purification: Purified by Protein A.

Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy.

Size: 100ul

Concentration: Lot dependent

Predicted Molecular Weight: 78


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot analysis of (1) HepG2 cell lysate; (2) Mouse spleen lysate; (3) Rat kidney lysate. Using ATG7 (bsm-61217R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


25 ug total protein per lane of various lysates (see on figure) probed with ATG7 monoclonal antibody, unconjugated (bsm-61217R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.