bsm-61195R [Primary Antibody]
Phospho-Histone H2B.1 (Thr129) Recombinant Antibody
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: Histone H2B.1

Modification Site: Thr129

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 851810

Swiss Prot: P02293

Source: KLH conjugated synthetic peptide derived from yeast Histone H2B.1 around the phosphorylation site of T129

Purification: Purified by Protein A.

Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Size: 100ul

Concentration: Lot dependent

Predicted Molecular Weight: 14


Cross Reactive Species: Yeast

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot analysis of Saccharomyces cerevisiae cell lysate treated with Methyl methanesulfonate. Using Phospho-Histone H2B.1 (Thr129) (bsm-61195R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.