bsm-61194R [Primary Antibody]
Phospho-Histone H2A (Ser129) Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Histone H2A

Modification Site: Ser129

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 852283

Swiss Prot: P04912

Source: KLH conjugated synthetic peptide derived from yeast Histone H2A around the phosphorylation site of S129

Purification: Purified by Protein A.

Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Core component of nucleosome which plays a central role in DNA double strand break (DSB) repair. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Size: 100ul

Concentration: Lot dependent

Predicted Molecular Weight: 14


Cross Reactive Species: Yeast

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot analysis of Saccharomyces cerevisiae treated with Methyl methanesulfonate. Using Phospho-Histone H2A (Ser129) (bsm-61194R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.