Host: Rabbit
Target Protein: Glutamine synthetase
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 2752
Swiss Prot: P15104
Source: KLH conjugated synthetic peptide derived from human Glutamine synthetase
Purification: Purified by Protein A.
Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: Lot dependent
Predicted Molecular Weight: 42
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with Glutamine synthetase monoclonal antibody, unconjugated (bsm-61160R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glutamine synthetase Monoclonal Antibody, Unconjugated(bsm-61160R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glutamine synthetase Monoclonal Antibody, Unconjugated(bsm-61160R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glutamine synthetase Monoclonal Antibody, Unconjugated(bsm-61160R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glutamine synthetase Monoclonal Antibody, Unconjugated(bsm-61160R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glutamine synthetase Monoclonal Antibody, Unconjugated(bsm-61160R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glutamine synthetase Monoclonal Antibody, Unconjugated(bsm-61160R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glutamine synthetase Monoclonal Antibody, Unconjugated(bsm-61160R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glutamine synthetase Monoclonal Antibody, Unconjugated(bsm-61160R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glutamine synthetase Monoclonal Antibody, Unconjugated(bsm-61160R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embeddedHuman Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glutamine synthetase Monoclonal Antibody, Unconjugated(bsm-61160R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embeddedHuman Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glutamine synthetase Monoclonal Antibody, Unconjugated(bsm-61160R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.