Host: Rabbit
Target Protein: HSP90 beta
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 3326
Swiss Prot: P08238
Source: KLH conjugated synthetic peptide derived from human Hsp90 beta
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: Lot dependent
Predicted Molecular Weight: 83
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Western blot analysis of (1)HeLa cell lysate;(2)Jurkat cell lysate. Using HSP90 beta (bsm-61128R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HSP90 beta) monoclonal Antibody, unconjugated (bsm-61128R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.