bsm-60882R
[Primary Antibody]
CD11b Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CD11b
Clonality: Monoclonal
Isotype: IgG
Purification: Purified by Protein A.
Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage: Store at 4°C for short term. Store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Background:
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
Size: 100ul
Concentration: Lot dependent
Predicted Molecular Weight: 125
Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
25 ug total protein per lane of various lysates (see on figure) probed with CD11b monoclonal antibody, unconjugated (bsm-60882R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.