VALIDATION IMAGES
Lane 1: 5% Input:Human MCF-7 cell lysates; Lane 2: Immunoprecipitation using rabbit monoclonal IgG homotypic control; Lane 3: Immunoprecipitation with bsm-60865R, probed with Acetyl Lysine monoclonal Antibody, Unconjugated (bsm-60865R) at 1:50 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HeLa cell lysates; Lane 2: Human NIH/3T3 cell lysates; Lane 3: Human BRL cell lysates; Lane 4: Mouse Liver tissue lysates; Lane 5: Mouse Kidney tissue lysates probed with Acetyl Lysine Monoclonal Antibody, Unconjugated (bsm-60865R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: PTM-105RM pre adorbed with 3 μ M synthetic Kac peptides; Lane 2: PTM-105RM pre adorbed with 3 μ M unmodified peptides probed with Acetyl Lysine Monoclonal Antibody, Unconjugated (bsm-60865R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.