Host: Rabbit
Target Protein: p53
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 7157
Swiss Prot: P04637
Source: KLH conjugated synthetic peptide derived from human P53
Purification: Purified by Protein A.
Storage Buffer: PBS, Glycerol, BSA
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(WB=1:500-2000)
IHC-P(IHC-P=1:50-500)
IHC-F(IHC-F=1:50-500)
IF(IF=1:20-50)
Predicted Molecular Weight: 43
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
- Zhang Kai. et al. Mitochondria-mediated apoptosis and endoplasmic reticulum stress are involved in the toxicity induced by copper in the porcine spleen. ENVIRON SCI POLLUT R. 2023 Aug;:1-12Read more>>
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (p53) Monoclonal Antibody, Unconjugated (bsm-60806R) at 1:500 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (p53) Monoclonal Antibody, Unconjugated (bsm-60806R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human ovarian cancer); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (p53) Monoclonal Antibody, Unconjugated (bsm-60806R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with p53 monoclonal antibody, unconjugated (bsm-60806R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.