Host: Rabbit
Target Protein: NSE
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 2026
Swiss Prot: P09104
Source: KLH conjugated synthetic peptide derived from human NSE
Purification: Purified by Protein A.
Storage Buffer: PBS, Glycerol, BSA
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications: WB(1:300-5000)
Predicted Molecular Weight: 48
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Cerebellum tissue lysates; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human U87MG cell lysates; Lane 7: Human HeLa cell lysates probed with NSE Monoclonal Antibody, Unconjugated (bsm-60786R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with NSE monoclonal antibody, unconjugated (bsm-60786R) at 1:3000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.