Host: Mouse
Target Protein: Nestin
Clonality: Monoclonal
Isotype: IgG2a/Kappa
Entrez Gene: 10763
Swiss Prot: P48681
Source: KLH conjugated synthetic peptide derived from human Nestin
Purification: Purified by Protein G.
Storage Buffer: PBS, Glycerol, BSA
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications: IHC-P(1:200-400)
Predicted Molecular Weight: 178
Cross Reactive Species: Human
Predicted Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded (human spleen ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Nestin) Monoclonal Antibody, Unconjugated (bsm-60784M) at 1:500 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Nestin) Monoclonal Antibody, Unconjugated (bsm-60784M) at 1:500 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Nestin) Monoclonal Antibody, Unconjugated (bsm-60784M) at 1:500 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.