bsm-60759R [Primary Antibody]
STIP1 Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: STIP1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 10963

Swiss Prot: P31948

Source: KLH conjugated synthetic peptide derived from human STIP1

Purification: Purified by Protein A.

Storage Buffer: PBS, Glycerol, BSA.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)

Predicted Molecular Weight: 63


Predicted Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue: Rat testis Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60759R


Tissue: human testis Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60759R


Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human A549 cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human 293T cell lysates; Lane 7: Human HepG2 cell lysates; Lane 8: Human Jurkat cell lysates probed with STIP1 Monoclonal Antibody, Unconjugated (bsm-60759R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.