Host: Rabbit
Target Protein: CD63
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 967
Swiss Prot: P08962
Source: KLH conjugated synthetic peptide derived from human CD63
Purification: Purified by Protein A.
Storage Buffer: PBS, Glycerol, BSA
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)
Predicted Molecular Weight: 26
Cross Reactive Species: Mouse
Predicted Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD63) Monoclonal Antibody, Unconjugated (bsm-60749R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD63) Monoclonal Antibody, Unconjugated (bsm-60749R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with CD63 monoclonal antibody, unconjugated (bsm-60749R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with CD63 monoclonal antibody, unconjugated (bsm-60749R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.