Host: Rabbit
Target Protein: PDCD6IP
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 10015
Swiss Prot: Q8WUM4
Source: KLH conjugated synthetic peptide derived from human PDCD6IP
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
Predicted Molecular Weight: 95
Predicted Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Ti Qin. et al. Size-Exclusion Chromatography-Based Extracellular Vesicles Size Subtyping and Multiplex Membrane Protein Profiling for Differentiating Gastrointestinal Cancer Prognosis. ANALYST. 2023 SepRead more>>
Tissue: Human breast cancer Section type: Formalin-fixed & Paraffinembedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60716R
Lane 1: Mouse Raw264.7 cell lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Human K562 cell lysates; Lane 4: Human A549 cell lysates probed with PDCD6IP Monoclonal Antibody, Unconjugated (bsm-60716R) at 1:10000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with PDCD6IP monoclonal antibody, unconjugated (bsm-60716R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.