Host: Rabbit
Target Protein: phospho-IKB Alpha (Ser32 / Ser36)
Modification Site: Ser32 / Ser36
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 4792
Swiss Prot: Q9Z1E3
Source: KLH conjugated synthetic peptide derived from human phospho-IKB Alpha (Ser32 / Ser36)
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
Predicted Molecular Weight: 35
Predicted Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Feng Gao. et al. Goat milk exosomal microRNAs alleviate LPS-induced intestinal inflammation in mice. INT J BIOL MACROMOL. 2024 May;268:131698Read more>>
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): HeLa, (+): HeLa+ Human α-TNF (20ng/ml, 30min) Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 36 kDa Observed MW: 36 kDa
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): C6, (+): C6+ Calyculin A (100nM, 30min) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 36 kDa Observed MW: 36 kDa
Tissue: (A) Rat kidney, (B) Rat kidney, then the tissue was incubated with alkaline phosphatase (50 U/ml) at 37℃ for 1 hour Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60711R
Tissue: (A) Mouse kidney, (B) Mouse kidney, then the tissue was incubated with alkaline phosphatase (50 U/ml) at 37℃ for 1 hour Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60711R