Host: Rabbit
Target Protein: CK18
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 3875
Swiss Prot: P05783
Source: KLH conjugated synthetic peptide derived from human CK18
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
Predicted Molecular Weight: 48
Predicted Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: MOLT-4 (Negative control), 2: HeLa, 3: HeLa+ staurosporine (1 μM, 4 h) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 48 kDa Observed MW: 48, 22 kDa
Tissue: Human breast Section type: Formalin-fixed & Paraffinembedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60699R
Tissue: Human lung adenocarcinoma Section type: Formalin-fixed & Paraffinembedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60699R