bsm-60698R [Primary Antibody]
p21 Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: p21

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 1026

Swiss Prot: P39689

Source: KLH conjugated synthetic peptide derived from human p21

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(WB=1:200-1000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)

Predicted Molecular Weight: 18


Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ming-Feng He. et al. Coptisine Inhibits Influenza Virus Replication by Upregulating p21. MOLECULES. 2023 Jan;28(14):5398Read more>>
  • Derya Erisik. et al. Differences and Similarities between Colorectal Cancer Cells and Colorectal Cancer Stem Cells: Molecular Insights and Implications. ACS OMEGA. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Xi-Kun Yuan. et al. Effects of Life-Long Exercise on Age-Related Inflammation, Apoptosis, Oxidative Stress, Ferroptosis Markers, and NRF2/KAEP 1/Klotho Pathway in Rat Kidneys. PHYSIOL RES. 2024 Aug 31;73(4):577-591Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p21 Monoclonal Antibody, Unconjugated(bsm-60698R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Thyroid Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p21 Monoclonal Antibody, Unconjugated(bsm-60698R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p21 Monoclonal Antibody, Unconjugated(bsm-60698R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with p21 monoclonal antibody, unconjugated (bsm-60698R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.