Host: Rabbit
Target Protein: phospho-BRD2 (Ser37)
Modification Site: Ser37
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 6046
Swiss Prot: P25440
Source: KLH conjugated synthetic peptide derived from human phospho-BRD2 (Ser37)
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1ug/ul
Applications: WB(1:300-5000)
Predicted Molecular Weight: 88
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:20000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HEK-293T, 2: HEK-293T + Calyculin A (100 nM, 60 min) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 88 kDa Observed MW: 110 kDa