Host: Rabbit
Target Protein: Histone H3 (Tri Methyl K27)
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 8350
Swiss Prot: P68431
Source: KLH conjugated synthetic peptide derived from human Histone H3 (Tri Methyl K27)
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1ug/ul
Applications: WB(1:300-5000)
Predicted Molecular Weight: 15
Predicted Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab:1: HeLa cytosol, 2: HeLa nuclear Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 15 KDa Observed MW: 15 KDa
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab:1: Mouse brain, 2: Rat brain, 3: Recombinant histone H3 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 15 KDa Observed MW: 15 KDa