Host: Rabbit
Target Protein: Histone H2A (Acetyl-Lys9)
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 3012
Swiss Prot: Q16777
Source: KLH conjugated synthetic peptide derived from human Histone H2A (Acetyl-Lys9)
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1ug/ul
Applications: WB(1:300-5000)
Predicted Molecular Weight: 14
Predicted Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4℃ overnight Secondary Ab: (-): MCF-7, (+): MCF-7+ sodium butyrate (50mM, 24hr) + trichostain A (500ng/ml, 4 hr) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 14 KDa Observed MW: 14 KDa
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4℃ overnight Secondary Ab: (-): NIH/3T3, (+): NIH/3T3+ trichostain A (1μM, 18 hr) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 14 KDa Observed MW: 14 KDa