Host: Rabbit
Target Protein: HES5
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 388585
Swiss Prot: Q5TA89
Source: KLH conjugated synthetic peptide derived from human HES5
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Size: 100ul
Concentration: 1ug/ul
Applications: WB(1:300-5000)
Predicted Molecular Weight: 18
Predicted Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:50000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Human kidney cancer, 2: Human liver, 3: Human lung, 4: Mouse liver, 5: Rat liver Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 18 kDa Observed MW: 15 kDa
25 ug total protein per lane of various lysates (see on figure) probed with HES5 monoclonal antibody, unconjugated (bsm-60648R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.