Host: Rabbit
Target Protein: phospho-AKT (Thr308)
Modification Site: Thr308
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 208
Swiss Prot: P31751
Source: KLH conjugated synthetic peptide derived from human phospho-AKT (Thr308)
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1ug/ul
Applications: WB(1:300-5000)
Predicted Molecular Weight: 56
Predicted Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
- ingjie Ge. et al. Integrated network toxicology, molecular docking, and in vivo experiments to elucidate molecular mechanism of aflatoxin B1 hepatotoxicity. ECOTOX ENVIRON SAFE. 2024 Apr;275:116278Read more>>
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: NIH/3T3, 2: NIH/3T3 + PDGF (100 ng/mL, 15 min) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 56 kDa Observed MW: 60 kDa