bsm-60645R [Primary Antibody]
phospho-AKT (Thr308) Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: phospho-AKT (Thr308)

Modification Site: Thr308

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 208

Swiss Prot: P31751

Source: KLH conjugated synthetic peptide derived from human phospho-AKT (Thr308)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 56


Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • ingjie Ge. et al. Integrated network toxicology, molecular docking, and in vivo experiments to elucidate molecular mechanism of aflatoxin B1 hepatotoxicity. ECOTOX ENVIRON SAFE. 2024 Apr;275:116278Read more>>
  • Wang Yunyun. et al. Iridoids rich fraction from Valeriana jatamansi Jones promotes axonal regeneration and motor functional recovery after spinal cord injury through activation of the PI3K/Akt signaling pathway. FRONT MOL NEUROSCI. 2024 May;17Read more>>
VALIDATION IMAGES

Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: NIH/3T3, 2: NIH/3T3 + PDGF (100 ng/mL, 15 min) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 56 kDa Observed MW: 60 kDa